A Simple, accurate, rapid, sensitive and precise RP-HPLC method has been developed for estimation of Hydrochlorothiazide and Metoprolol Succinate in tablet dosage form using PDA detector. A Reverse phase Thermo Hypersil ODS c18 column (250mm × 4.6 mm I.D.,× 3µ particle size) with mobile phase consisting of Acetonitrile and Triethylamine buffer 100:900(v/v) [Ph 3±0.02 adjusted with orthophosphoric acid] was used. The flow rate was 1.5ml/min and the effluents were monitored at 238nm, and the injection volume was 10µl. The separation was performed at ambient temperature. Retention time of Hydrochlorothiazide and Metoprolol Succinate was found to be 1.9 and 3.8 minutes respectively. The linearity of the methods was found to be 50-150µg/ml for Hydrochlorothiazide and 50-150µg/ml for Metoprolol Succinate. The correlation co-efficient of Hydrochlorothiazide was found to be 0.9996 and the correlation co-efficient of Metoprolol Succinate was found to be 0.9998. The % Recoveries of Hydrochlorothiazide and Metoprolol Succinate were found to be between 100-102%. The system suitability parameters such as Theoretical plates and Tailing factor were found to be 3250, 1.4 and 4742, 1.1 respectively for Hydrochlorothiazide and Metoprolol Succinate. Hence the proposed method was successfully applied for routine quality control analysis of Hydrochlorothiazide and Metoprolol in combined tablet dosage forms. This method was validated according to ICH guidelines.
Loading....